These tulasi beads were carved by one of Srila Prabhupada's disciples in Hawaii, from tulasi grown there. Guru Kripa Das offered these tulasi beads to Srila Prabhupada and Prabhupada chanted on them. - Sri Sri Krishna Balarama Temple, Vrindavan.
Reference:Thesetulasibeads were carved by one of Srila Prabhupada's disciples in Hawaii,